Fish Functional Genomics IIM-CSIC platform (GFP-IIM-CSIC) at FishBioTech Lab aims to facilitate research in animal models of aquatic vertebrates offering advice in several cutting-edge technologies of molecular biology, genetics and histology, using zebrafish (Danio rerio) as a model species. The service offers the processing and analysis of the samples as well as their facilities and equipment, so that customers can process and analyze their samples themselves (self-service) always in strict compliance with the existing platform regulations.

 

 

Services

The GFP-IIM-CSIC platform (FishBioTech Lab) offers technical support to users in several cutting-edge technologies of molecular biology, genetics and histology using zebrafish (Danio rerio) as a model species.

The tasks performed by the service are the following:

  • Micro-injection of DNA, morpholino or mRNA in zebrafish embryos.

  • Generation of stable zebrafish mutant and transgenic lines.

  • CRISPR/Cas9; Tol2Kit and BAC-transgenesis Technologies.

  • Maintenance of transgenic and mutant zebrafish lines .

  • Delivery of embryos, larvae, juveniles and adults (WT, transgenic and mutant fish).

  • Whole mount” in situ hybridization and inmunohistochemistry tecniques.

  • Genotyping: extraction and quantification of DNA from embryos or fish fin.

  • Isolation, purification and quantification of nucleic acids “Qubit quantification”.

  • Synthesis of cDNA, “Capped” -mRNA and RNA probes.

  • Cloning of genes of interest in plasmid vectors. Gateway recombination cloning.

  • RT-PCR (Thermocycler (PCR)) (self-service option).

  • Real-Time qPCR (SYBRGREEN) and data analysis (self-service option).

 

Contact

 

Josep Rotllant Moragas

e-mail: rotllant@iim.csic.es

Tel.: 986231930 ext. 860288

 

Paula Suarez-Bregua

e-mail: paulasuarez@iim.csic.es

Tel.: 986231930 ext. 860289

 

Web: http://fishbiotech.csic.es
Users can contact the service staff via telephone or email and fill in the corresponding service request form.
Download service request form.
Hours of attention to public: 8h to 15h.

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